A joint project of the Graduate School, Peabody College, and the Jean & Alexander Heard Library

Title page for ETD etd-12202018-104355


Type of Document Dissertation
Author Wepy, James Anthony
URN etd-12202018-104355
Title Lysophospholipases as Mediators of Bioactive Lipid Metabolism and Signaling
Degree PhD
Department Chemistry
Advisory Committee
Advisor Name Title
Lawrence J. Marnett Committee Chair
Alan R. Brash Committee Member
Brian O. Bachmann Committee Member
Gary A. Sulikowski Committee Member
Keywords
  • protein palmitoylation
  • neuritogenesis
  • neuron
  • CRISPR
  • prostaglandin
  • signaling
  • lipid
  • lysophospholipid
  • lysophospholipase
Date of Defense 2018-12-14
Availability unrestricted
Abstract
Lysophospholipase enzymes, lysophospholipase A1 (LYPLA1) and lysophospholipase A2 (LYPLA2), are serine hydrolases responsible for the metabolism of three separate classes of bioactive lipid substrates, including lysophospholipids, prostaglandin glyceryl esters (PGG), and the S-palmitoylcysteine post-translational modification of proteins. This diverse range of substrates suggests a regulatory role of LYPLAs in a number of associated physiological processes. To understand the structural determinants of LYPLA substrate selectivity, we employed x-ray crystallography resulting in the first crystal structure of LYPLA2. These structural investigations reveal key structural differences between the enzymes, specifically in potential substrate-binding regions and sites of post-translational modification. Site-directed mutagenesis was employed to investigate these structural features with recombinant protein. Furthermore, Lypla1-/-, Lypla2-/- and double knockout neuroblastoma cell models were developed using CRISPR-Cas9 technology to investigate the activity of LYPLAs toward each type of substrate in a cellular setting. Extensive lipidomics was performed to identify changes in lipid metabolism and signaling, and LYPLA-dependent phenotypic changes were characterized in the knockout cells. Acyl-protein thioesterase activity in these cells was also assessed using alkynyl-palmitic acid and click chemistry to label palmitoylated proteins. These data suggest modest increases in global protein palmitoylation in double knockout cells and preliminary experiments have identified multiple lipoprotein substrates of LYPLAs. Here we describe the first kinetic and cellular analyses of LYPLAs’ ability to hydrolyze a range of PGG, lysophospholipid, and lipoprotein substrates. Collectively, these data provide the first structural interpretation of the molecular interactions of LYPLAs with their lipid substrates.
Files
  Filename       Size       Approximate Download Time (Hours:Minutes:Seconds) 
 
 28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access 
  Wepy.pdf 109.25 Mb 08:25:47 04:20:07 03:47:36 01:53:48 00:09:42

Browse All Available ETDs by ( Author | Department )

If you have more questions or technical problems, please Contact LITS.