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Title page for ETD etd-12042008-144306

Type of Document Master's Thesis
Author Garmendia, Craig Alexander
Author's Email Address craig.garmendia@gmail.com
URN etd-12042008-144306
Title In vitro replication studies of oligonucleotides containing site-specific DNA adducts in frameshift-prone sequences
Degree Master of Science
Department Chemistry
Advisory Committee
Advisor Name Title
Carmelo J. Rizzo Committee Chair
F. Peter Guengerich Committee Member
Ned A. Porter Committee Member
  • Oligonucleotides -- Synthesis
  • Tandem Mass Spectrometry
  • Frameshift
  • DNA Adducts
  • Acrolein
  • Crotonaldehyde
  • Chemical carcinogenesis
Date of Defense 2008-12-04
Availability unrestricted
Exposure to endogenous and exogenous chemicals can lead to modification of DNA and potentially lead to cancer. Frameshift mutations may be a consequence of these modifications to DNA. Oligonucleotides were synthesized that contain a site specific a site specific C8-deoxyguanosine adduct 2-amino-3-methylnaphtho[1,2-d]imidazole (CCIQ), which is an analogue of the food mutagen 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), and the adducts R- and S-á-methyl-ã-hydroxy-1,N2-propano-2’-deoxyguaosine and ã-hydroxy-1,N2-propano-2’-deoxyguanosine in frameshift prone sequences. In vitro replication studies were conducted to examine which nucleotide was incorporated opposite the adducts by a variety of DNA polymerases. The sequence and yields of the extension products were determined using tandem mass spectrometry.
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