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Title page for ETD etd-11302010-140606

Type of Document Dissertation
Author Carmody, Sean Robert
Author's Email Address sean.r.carmody@vanderbilt.edu
URN etd-11302010-140606
Title Analysis of the mRNA-binding protein Nab2 during the nuclear export mechanism
Degree PhD
Department Cell and Developmental Biology
Advisory Committee
Advisor Name Title
Kathleen Gould Committee Chair
Anna Means Committee Member
Daniel Liebler Committee Member
Ronald Emeson Committee Member
  • heat shock response
  • mRNA export
  • Nab2
Date of Defense 2010-09-22
Availability unrestricted
This project is concerned with understanding the role of the mRNA-binding protein Nab2 in the process of nuclear mRNA export. In this dissertation I characterize changes that occur to Nab2’s structure and localization during cellular response to increased growth temperatures, known as the heat shock response. I show that Nab2 is both phosphorylated by the MAP kinase Slt2, and re-localizes to discrete intranuclear foci during heat shock. Further, I show that these changes are involved in nuclear retention of mRNA during heat shock, and that loss of the Nab2 kinase results in lack of this retention. In summary, this work demonstrates that Nab2, and the MAP kinase Slt2, are involved in nuclear retention of mRNA during heat shock.

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