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Title page for ETD etd-11192015-095602

Type of Document Dissertation
Author Millsap, Amy Danae
URN etd-11192015-095602
Title Translesion Synthesis of N2 and C8-Deoxyguanosine Adducts of the Dietary Mutagen 2-Amino-3-methylimidazo-[4,5-f]-quinoline (IQ): Participation in Observed Mutagenic Spectra
Degree PhD
Department Chemistry
Advisory Committee
Advisor Name Title
Carmelo J. Rizzo, Ph.D. Committee Chair
Brandt F. Eichman Committee Member
Martin Egli, Ph.D. Committee Member
Michael P. Stone Committee Member
  • DNA Adducts
  • Translesion Synthesis
  • 2-Amino-3-methylimidazo-[4
  • 5-f]-quinoline
  • Heterocyclic Amines
  • DNA Polymerases
Date of Defense 2015-11-03
Availability unrestricted
2-Amino-3-methylimidizo-[4,5-ʄ]-quinoline (IQ), a potent dietary mutagen, forms DNA adducts at the N&178; and C8-positions of 2ʹ-deoxyguanosine. IQ induces G to A transitions and G to T transversions in eukaryotic cells. The in vitro replication of the N&178;-dG-IQ and C8-dG-IQ adducts was examined by translesion DNA polymerases to determine their contributions to the observed mutagenic spectra. Our results implicate DNA polymerases kappa and eta in the error-free bypass of N2- and C8-dG-IQ adducts, respectively. In addition, DNA polymerase combinations eta/zeta and kappa/zeta are implicated in the mis-incorporation of A and T.

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