A joint project of the Graduate School, Peabody College, and the Jean & Alexander Heard Library

Title page for ETD etd-11162018-123823


Type of Document Dissertation
Author Strnad, Jessica Ann
URN etd-11162018-123823
Title Sequence, Structure, and Function Relationships of Human Antibodies
Degree PhD
Department Microbiology and Immunology
Advisory Committee
Advisor Name Title
Mark Denison Committee Chair
Andrew J. Link Committee Member
James E. Crowe, Jr. Committee Member
James W. Thomas, II Committee Member
Jens Meiler Committee Member
John Anthony Capra Committee Member
Jonathan M. Irish Committee Member
Keywords
  • B cell
  • structural modeling
  • next generation sequencing
  • antibody repertoire
  • influenza
  • Rosetta
Date of Defense 2018-12-15
Availability restrictone
Abstract
The human adaptive immune system is mediated in part by B cells, which produce antibodies to protect the body from infection. Antibodies are protein molecules responsible for recognizing and binding pathogenic targets (i.e., antigens) to mediate effective neutralization of the microorganism. In recent years, emerging technologies such as next generation sequencing and computational structure prediction have had a substantial impact on studies of human antibodies. These technologies have improved our understanding of antibody sequence, structure and function relationships. The development of a broadly protective anti-influenza antibody response following vaccination was tracked over time using next generation sequencing. Knowledge-based restraints calculated from analysis of conserved structural motifs in antigen-binding antibody protein loops improved the accuracy of antibody structural predictions using the Rosetta software suite for macromolecular modeling. Combining these sequencing techniques with structure prediction allowed for the development of a novel method for structure-based discovery of functional antibodies from human donors.
Files
  Filename       Size       Approximate Download Time (Hours:Minutes:Seconds) 
 
 28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access 
[campus] Finn.pdf 12.43 Mb 00:57:33 00:29:36 00:25:54 00:12:57 00:01:06
[campus] indicates that a file or directory is accessible from the campus network only.

Browse All Available ETDs by ( Author | Department )

If you have more questions or technical problems, please Contact LITS.