A joint project of the Graduate School, Peabody College, and the Jean & Alexander Heard Library

Title page for ETD etd-10222018-135625


Type of Document Dissertation
Author Kara, Nergis
Author's Email Address nergis.kara@vanderbilt.edu
URN etd-10222018-135625
Title microRNA Function in Zebrafish Development and Regeneration
Degree PhD
Department Biological Sciences
Advisory Committee
Advisor Name Title
Douglas G. McMahon Committee Chair
James G. Patton Committee Member
John A. Capra Committee Member
Wenbiao Chen Committee Member
Keywords
  • zebrafish
  • microRNA
  • development
  • regeneration
  • retina
  • Müller glia
Date of Defense 2018-07-27
Availability unrestricted
Abstract
microRNAs (miRNAs) are a family of highly conserved small noncoding RNAs that post transcriptionally regulate gene expression and play important roles in many cellular processes during development and regeneration. The work presented in this thesis focuses on discovering functions of miRNAs in craniofacial development and retina regeneration in zebrafish. Chapter II describes the role of miR-27 in regulating chondrogenesis during pharyngeal morphogenesis. Chondrogenesis in the pharyngeal arches involves differentiation of cranial neural crest cells into chondrocytes, tightly regulated by temporal intracellular and extracellular cues. miR-27 is highly enriched in the pharyngeal arches and acts as a positive regulator of chondrogenesis by controlling the activity of Focal Adhesion Complex. Chapter III focuses on retina regeneration in adult zebrafish and describes how miR-216 regulates the initiation of Müller glia (MG) dependent retina regeneration. Unlike the adult mammalian retina, MG in the adult zebrafish retina are able to dedifferentiate into a ‘stem cell’-like state and give rise to multipotent progenitor cells upon retinal damage. miR-216a is downregulated in MG after constant intense light lesioning allowing upregulation of the H3K79 methyltransferase Dot1l which is required for MG reprogramming and proliferation downstream of miR-216a. Chapter IV presents a genome-wide transcriptome analysis of MG dedifferentiation during retina regeneration serving as a resource for the identification of potential therapeutic targets.
Files
  Filename       Size       Approximate Download Time (Hours:Minutes:Seconds) 
 
 28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access 
  Kara.pdf 15.73 Mb 01:12:49 00:37:27 00:32:46 00:16:23 00:01:23

Browse All Available ETDs by ( Author | Department )

If you have more questions or technical problems, please Contact LITS.