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Title page for ETD etd-07182012-102603

Type of Document Master's Thesis
Author Matthews, Gage Franklin
URN etd-07182012-102603
Title Determination of local movements of the FG-loop and α-helix of sodium-calcium exchanger NCX1.1 upon binding of calcium ions using EPR
Degree Master of Science
Department Chemical and Physical Biology
Advisory Committee
Advisor Name Title
Albert Beth Committee Chair
Chuck Sanders Committee Member
Hassane Mchaourab Committee Member
  • calcium
  • cardiac
  • ion transporters
  • structural biology
  • electron paramagnetic resonance
Date of Defense 2012-04-18
Availability unrestricted
The structural analysis of the sodium-calcium exchanger NCX1.1 is presented in this thesis. I explore the background information and previous research detailing NCX1.1 and its mechanism. I also explain some of the technical and important details of the primary method used in this research, which is electron paramagnetic resonance (EPR). Using EPR, I found that a small alpha-helix of NCX1.1 undergoes no conformational change upon the binding of calcium ions to the protein. Also, the results suggest that another region, a small unstructured loop, may undergo some change when calcium ions are bound. This opens the door to future research on this protein and the mechanism of its activity.
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