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Title page for ETD etd-06012010-101403

Type of Document Dissertation
Author Harry, Stephen Reese
Author's Email Address sreeseharry@hotmail.com
URN etd-06012010-101403
Title Bioanalytical Approaches to Chemical Biology
Degree PhD
Department Chemistry
Advisory Committee
Advisor Name Title
David W Wright Committee Chair
Brian O. Bachmann Committee Member
Eric P Skaar Committee Member
John A McLean Committee Member
  • metabolomics
  • nanoparticle
  • HNE
  • melanoma
  • mRNA imaging
  • hemozoin
Date of Defense 2010-05-11
Availability unrestricted
As the field of chemical biology evolves, so does the development of novel bioanalytical techniques capable of accurately measuring biochemical composition and regulation. This dissertation explores three aspects of chemical biology using validated analytical methodology coupled with novel nanoscale intracellular detection probes. Using LC tandem mass spectrometry, identification and quantification of hydroxylated fatty acids associated with S. mansoni hemozoin was achieved. Additionally, the metabolic impact of 4-hydroxynonenal toxicity on macrophage activation was examined using multianalyte microphysiometry which confirmed a concentration and temporal inhibition of overall cellular metabolism. Utilizing the inherent characteristics of DNA-coated gold nanospheres, a new mRNA detection probe was developed that positively identifies tyrosinase-expressing melanoma cells. The broad scope of this work explores the use of new and emerging bioanalytical techniques and reagents that will be requisite for understanding biochemical activity at the single-cell level.
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