A joint project of the Graduate School, Peabody College, and the Jean & Alexander Heard Library

Title page for ETD etd-04092019-232027


Type of Document Dissertation
Author Anderson, Erica Joan Pruett
Author's Email Address erica.jp.anderson@gmail.com
URN etd-04092019-232027
Title Roles of Melanocortin-4 Receptor Signaling Partner Kir7.1 in Energy Homeostasis
Degree PhD
Department Molecular Physiology and Biophysics
Advisory Committee
Advisor Name Title
Danny Winder Committee Chair
Heidi Hamm Committee Member
Mark Magnuson Committee Member
Roger Colbran Committee Member
Keywords
  • POMC
  • AgRP
  • signaling modality
  • obesity
  • melanocortin
  • MC4R
  • Kir7.1
  • Kcnj13
Date of Defense 2019-02-28
Availability unrestricted
Abstract
Erica JP Anderson

Dissertation under the direction of Roger D Cone, PhD

Energy stores in fat tissue are determined in part by the activity of the hypothalamic neurons expressing the melanocortin-4 receptor (MC4R). Even partial reduction in MC4R expression levels in mice, rats, or humans produces hyperphagia and morbid obesity. Thus it is of great interest to understand the molecular basis of neuromodulation by the MC4R. The MC4R is a G-protein coupled receptor (GPCR) that signals efficiently through Gs, and this signaling pathway is essential for normal MC4R function in vivo. However, previous data from hypothalamic slice preparations indicated that activation of the MC4R depolarized neurons through G protein-independent regulation of the ion channel Kir7.1. Herein a panel of mouse models are used to study the physiologic and pharmacologic effects of loss of Gs and Kir7.1. Deletion of Kcnj13, the gene encoding Kir7.1, specifically from MC4R neurons produced resistance to melanocortin peptide-induced depolarization of MC4R PVN neurons in brain slices, resistance to the sustained anorexic effects of exogenously administered melanocortin peptides, late onset obesity, increased linear growth, and glucose intolerance. Some MC4R-mediated phenotypes appeared intact, including AgRP-induced stimulation of food intake, and MC4R-mediated induction of PYY release from intestinal L cells. Thus, a subset of the consequences of MC4R signaling in vivo appear dependent on expression of the Kir7.1 channel in MC4R cells.

Files
  Filename       Size       Approximate Download Time (Hours:Minutes:Seconds) 
 
 28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access 
  DissertationEJPA.v.02.pdf 11.70 Mb 00:54:10 00:27:51 00:24:22 00:12:11 00:01:02

Browse All Available ETDs by ( Author | Department )

If you have more questions or technical problems, please Contact LITS.