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Title page for ETD etd-03312008-162553

Type of Document Dissertation
Author Walker III, William Benjamin
Author's Email Address william.b.walker@vanderbilt.edu
URN etd-03312008-162553
Title On the nature of the sensory arrestins of the dipteran insects Anopheles gambiae and Drosophila melanogaster
Degree PhD
Department Neuroscience
Advisory Committee
Advisor Name Title
Vsevolod Gurevich Committee Chair
Kendal Scot Broadie Committee Member
Laurence J Zwiebel Committee Member
Todd R Graham Committee Member
  • Anopheles gambiae
  • Arrestin
  • Drosophila melanogaster
  • Olfaction
  • Desensitization
  • Smell -- Molecular aspects
  • Insects -- Physiology
Date of Defense 2008-03-17
Availability unrestricted
This project is concerned with the functional roles of the sensory arrestin genes, arr1 and arr2, in the regulation of dipteran olfactory signal transduction. Specifically, I have examined the sensory arrestins of the fruit fly, Drosophila melanogaster, and the malaria vector mosquito, Anopheles gambiae (An. gambiae). I have demonstrated that transgenic expression of An. gambiae arr1 in the antennae of arr1 mutant fruit flies sufficiently rescues olfactory deficits in these mutant flies. This implies functional orthology between the fruit fly and mosquito arr1 homologues. I also sought to examine spatial and temporal characteristics of these genes in the antennae of D. melanogaster. I attempted to identify the spatial expression patterns of arr1 and arr2 mRNA in antennal neurons via fluorescence in situ hybridization. However, the expression levels of these genes lie below the threshold of detection for the applied methodology, as no signal corresponding to the expression of these genes was detected. I utilized time-controlled induction of wild-type arr1 transgene paradigms in arr1 mutant fruit flies to assess the role of this gene as it pertains to the temporal dynamics of the effects of this gene on the olfactory system of the fly. The results of these studies are inconclusive and require further experimentation.
  Filename       Size       Approximate Download Time (Hours:Minutes:Seconds) 
 28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access 
  01introductory.pdf 137.54 Kb 00:00:38 00:00:19 00:00:17 00:00:08 < 00:00:01
  02Chapter1.pdf 1.10 Mb 00:05:04 00:02:36 00:02:16 00:01:08 00:00:05
  03Chapter2.pdf 1.94 Mb 00:08:58 00:04:36 00:04:02 00:02:01 00:00:10
  04Chapter3.pdf 749.10 Kb 00:03:28 00:01:47 00:01:33 00:00:46 00:00:03
  05Chapter4.pdf 1.07 Mb 00:04:56 00:02:32 00:02:13 00:01:06 00:00:05
  06Chapter5.pdf 113.99 Kb 00:00:31 00:00:16 00:00:14 00:00:07 < 00:00:01
  07References.pdf 214.32 Kb 00:00:59 00:00:30 00:00:26 00:00:13 00:00:01

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