| Type of Document |
Master's Thesis |
| Author |
Miller, Sinead Emily
|
| Author's Email Address |
sinead.e.miller@vanderbilt.edu |
| URN |
etd-11202015-152348 |
| Title |
Colistin-Functionalized Nanoparticles for the Rapid Capture of Acinetobacter baumannii |
| Degree |
Master of Science |
| Department |
Biomedical Engineering |
| Advisory Committee |
| Advisor Name |
Title |
| Todd D. Giorgio |
Committee Chair |
| Frederick R. Haselton |
Committee Member |
|
| Keywords |
- Lipopolysaccharide
- Gram-negative
- Multi-Drug Resistance
- Gold
|
| Date of Defense |
2015-11-19 |
| Availability |
unrestricted |
Abstract
Gold nanoparticles (AuNPs) were functionalized for rapid binding of Acinetobacter baumannii (A. baumannii), a Gram-negative bacterium. AuNPs were functionalized with colistin (Col), a polycationic antibiotic, using a two-step self-assembly process, in which heterobifunctional polyethylene glycol (PEG) was used as a linker. Colistin was successfully conjugated to the AuNPs (Col-PEG-AuNP), as validated by dynamic light scattering (DLS) and proton nuclear magnetic resonance (H1 NMR). Images taken using a scanning transmission electron microscope (STEM), in combination with x-ray energy dispersive spectroscopy (EDS), confirmed binding of Col-PEG-AuNPs to the cell wall of A. baumannii. Results generated from a rapid binding assay indicated that the reaction rate of Col-PEG-AuNP complexation with A. baumannii reached half-maximum saturation in approximately 7 minutes. Quantitative measurement of the kinetics of Col-PEG-AuNP binding to A. baumannii is essential to inform the design of colistin-functionalized magnetic nanoparticles for magnetic separation of nanoparticle-bound A. baumannii.
|
| Files |
| Filename |
Size |
Approximate Download Time
(Hours:Minutes:Seconds)
|
| 28.8 Modem |
56K Modem |
ISDN (64 Kb) |
ISDN (128 Kb) |
Higher-speed Access |
| |
SineadMiller.pdf |
8.12 Mb |
00:37:36 |
00:19:20 |
00:16:55 |
00:08:27 |
00:00:43 |
|
