Type of Document	Dissertation
Author	Millsap, Amy Danae
URN	etd-11192015-095602
Title	Translesion Synthesis of N2 and C8-Deoxyguanosine Adducts of the Dietary Mutagen 2-Amino-3-methylimidazo-[4,5-f]-quinoline (IQ): Participation in Observed Mutagenic Spectra
Degree	PhD
Department	Chemistry
Advisory Committee	Advisor Name Title Carmelo J. Rizzo, Ph.D. Committee Chair Brandt F. Eichman Committee Member Martin Egli, Ph.D. Committee Member Michael P. Stone Committee Member
Keywords	DNA Adducts Translesion Synthesis 2-Amino-3-methylimidazo-[4 5-f]-quinoline Heterocyclic Amines DNA Polymerases
Date of Defense	2015-11-03
Availability	unrestricted
Abstract 2-Amino-3-methylimidizo-[4,5-ʄ]-quinoline (IQ), a potent dietary mutagen, forms DNA adducts at the N&178; and C8-positions of 2ʹ-deoxyguanosine. IQ induces G to A transitions and G to T transversions in eukaryotic cells. The in vitro replication of the N&178;-dG-IQ and C8-dG-IQ adducts was examined by translesion DNA polymerases to determine their contributions to the observed mutagenic spectra. Our results implicate DNA polymerases kappa and eta in the error-free bypass of N2- and C8-dG-IQ adducts, respectively. In addition, DNA polymerase combinations eta/zeta and kappa/zeta are implicated in the mis-incorporation of A and T.
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