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Title page for ETD etd-12102010-133635


Type of Document Master's Thesis
Author Trantum, Joshua Robert
Author's Email Address Joshua.R.Trantum@vanderbilt.edu
URN etd-12102010-133635
Title Low-resource diagnostic assay based on disruption of radial flow in an evaporating drop
Degree Master of Science
Department Biomedical Engineering
Advisory Committee
Advisor Name Title
Frederick R. Haselton Committee Chair
David W. Wright Committee Member
Keywords
  • microparticle
  • coffee stain phenomenon
  • malaria
  • malaria diagnostic
  • low resource
  • diagnostic
  • diagnostic assay
  • low resource diagnostic assay
  • coffee ring phenomenon
  • nanoparticle
  • radial flow
Date of Defense 2010-12-10
Availability unrestricted
Abstract
More than 700,000 deaths are attributed to malarial infection annually in the rural areas of Sub-Saharan Africa. Effective and reliable early detection is inadequate with currently available technology. Development of a low-cost, sensitive and simple-to-use diagnostic assay appropriate for the point-of-care setting is a critical component of malaria containment efforts. This work has demonstrated a diagnostic assay based on the same mechanism that causes a ring to form in an evaporating coffee drop. This novel method for noninstrumented signal visualization is potentially well-suited for low-resource applications that require a simple-to-use, low cost method for pathogen detection. After exploring and optimizing basic design parameters, we demonstrated proof-of-principle for malaria testing by detecting poly-l-histidine, a biomolecular mimic of pfHRP2. The proposed assay was found to have a limit of detection in the mid-nanomolar range, approximately one order of magnitude higher than the clinically relevant range.
Files
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  trantum.pdf 16.25 Mb 01:15:14 00:38:41 00:33:51 00:16:55 00:01:26

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