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Title page for ETD etd-12072012-222004


Type of Document Master's Thesis
Author Todd, Erik Michael
Author's Email Address ErikMTodd@Gmail.com
URN etd-12072012-222004
Title Method development for routine imaging mass spectrometry of single cells grown in culture using transmission geometry MALDI mass spectrometry
Degree Master of Science
Department Chemistry
Advisory Committee
Advisor Name Title
Richard M. Caprioli Committee Chair
Carmello Rizzo Committee Member
David L. Black Committee Member
David L. Hachey Committee Member
Keywords
  • Transmission Geometry
  • Single Cell
  • Imaging Mass Spectrometry
  • MALDI
  • Lipid
Date of Defense 2012-09-07
Availability unrestricted
Abstract
Routine analysis of cells smaller than 10 microns is a challenging task. Instruments capable of single cell analysis require advanced modifications to both laser optics and optimized instrument sensitivity to allow for the detection of the requisite numbers of molecules in a single cell.. This study applies MALDI, along with key sample preparation techniques, to the analysis of single cells. A novel transmission geometry source was used along with specific sample preparation steps required for high spatial resolution analysis of a single cell
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