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Title page for ETD etd-12072007-113408


Type of Document Dissertation
Author Khatib-Shahidi, Sheerin
URN etd-12072007-113408
Title Direct analysis of endogenous and exogenous compounds in whole-animal tissue sections by imaging MALDI mass spectrometry
Degree PhD
Department Chemistry
Advisory Committee
Advisor Name Title
Richard M. Caprioli Committee Chair
David Wright Committee Member
Dennis Hallahan Committee Member
Michael P. Stone Committee Member
Keywords
  • Matrix assisted laser desorption-ionization
  • drug
  • mass spectrometry
  • whole-body
  • MALDI
  • tissue
  • imaging
  • Drug development -- Methodology
  • Tissues -- Analysis
  • Drugs -- Metabolism
Date of Defense 2007-09-07
Availability unrestricted
Abstract
In recent years, technological advances have improved our understanding of disease and infection at the molecular level. Genomic and proteomic studies have identified a large number of potential therapeutic targets for which combinatorial libraries have produced many more possible remedial compounds. Despite this exponential increase in knowledge and productivity, the drug discovery and development process is still a lengthy journey with high attrition rates for lead compounds. In this dissertation, a novel imaging MALDI mass spectrometry methodology was developed and applied to detect the label-free distribution of drug and metabolite compounds across whole-animal tissue sections. This work has lead to a technology that can facilitate the rapid screening and evaluation of compounds earlier in the drug discovery and development process, ultimately saving time and money.

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