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Title page for ETD etd-12072006-092733


Type of Document Dissertation
Author Gerdon, Aren Elliott
Author's Email Address a.gerdon@vanderbilt.edu
URN etd-12072006-092733
Title Peptide Epitope Functionalized Monolayer-Protected Clusters for Life Science Applications
Degree PhD
Department Chemistry
Advisory Committee
Advisor Name Title
David E. Cliffel Committee Chair
David W. Wright Committee Member
Eva Harth Committee Member
Ned Porter Committee Member
Raymond Mernaugh Committee Member
Keywords
  • quartz crystal microbalance
  • peptide
  • antigen mimic
  • immunosensor
  • nanocluster
  • Nanoparticles -- Analysis
  • Quartz crystal microbalances
  • Antigenic determinants -- Analysis
  • Biosensors
  • Mimicry (Chemistry)
Date of Defense 2006-12-05
Availability unrestricted
Abstract
Developments at the interface between biology and nanoscale materials are crucial for the success of bionanotechnology in academic and industrial endeavors. An early development was made in assembling biological antibodies with inorganic nanoclusters through peptide-based interfaces. The thesis of this work is based on the idea that better interfaces can be assembled using monolayer-protected cluster scaffolds presenting peptide epitopes in a physiologically-relevant conformation. Significant progress towards this goal has resulted in sophisticated antigen mimics that make use of epitope primary structure as well as secondary structure. Nanoparticle-based antigen mimics were shown to be antigenic as well as immunogenic using monoclonal antibodies, mouse model studies, and an effective immunosensor based on quartz crystal microbalance technology. This project has provided a foundation for continued work on QCM-based pathogen detection, nanoparticle-based vaccines, nanoparticle-based sensor calibrants, and advanced immuno-materials interfaces.
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