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Title page for ETD etd-12052007-212904


Type of Document Dissertation
Author Burnett, Atuhani Seth
Author's Email Address atuhani_seth@hotmail.com
URN etd-12052007-212904
Title The role of RNA in the packaging APOBEC3G into human immunodeficiency virus type 1 particles
Degree PhD
Department Microbiology and Immunology
Advisory Committee
Advisor Name Title
Mark Denison Committee Chair
Christopher Aiken Committee Member
Earl Ruley Committee Member
James Patton Committee Member
Paul Spearman Committee Member
Keywords
  • confocal
  • FRET
  • NC
  • APOBEC3G
  • HIV
  • assembly
  • fluorescent
Date of Defense 2007-08-06
Availability unrestricted
Abstract
APOBEC3G is a cytidine deaminase that inhibits HIV replication at a post-entry step in replication. APOBEC3G must be incorporated into the virus particle during assembly in order to inhibit HIV during the next round of replication. We defined the highly basic region between the two zinc binding domains in the nucleocapsid subunit of the viral Gag protein as a crucial packaging domain for APOBEC3G. Using a novel fluorescent resonance energy transfer (FRET) assay to detect APOBEC multimers, we found that APOBEC3G is packaged as a multimer bound to packaged RNA. We demonstrate for the first time that APOBEC multimers complexed with RNA are specifically recruited to the plasma membrane site of assembly by Gag.
Files
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