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Title page for ETD etd-11252013-164025


Type of Document Master's Thesis
Author Martin, John Robert
Author's Email Address john.r.martin@vanderbilt.edu
URN etd-11252013-164025
Title Synthesis of a Porous, Biocompatible Tissue Engineering Scaffold Selectively Degraded by Cell-Generated Reactive Oxygen Species
Degree Master of Science
Department Biomedical Engineering
Advisory Committee
Advisor Name Title
Craig L. Duvall Committee Chair
Scott A. Guelcher Committee Member
Keywords
  • polyurethane
  • oxidation
  • biodegradation
  • macrophage
  • wound healing
  • scaffold
Date of Defense 2013-12-11
Availability unrestricted
Abstract
Biodegradable tissue engineering scaffolds are commonly fabricated from poly(lactide-co-glycolide) (PLGA) or similar polyesters that degrade by hydrolysis. PLGA hydrolysis generates acidic byproducts that trigger an accelerated, autocatalytic degradation mechanism that can create mismatched rates of biomaterial breakdown and tissue formation. Reactive oxygen species (ROS) are naturally produced by cells, and induction of inflammation and ROS is an inevitable in vivo response to biomaterial implantation. Thus, polymeric biomaterials that are selectively degraded by cell-generated ROS may have potential for creating scaffolds with better-matched rates of tissue in-growth and cell-mediated scaffold biodegradation. To explore this approach, a series of novel poly(thioketal) (PTK) urethane (PTK-UR) biomaterial scaffolds that degrade specifically by an ROS-dependent mechanism were synthesized. Unlike poly(ester-urethane) (PEUR) scaffolds, the PTK-UR scaffolds were stable under aqueous conditions out to 25 weeks but were selectively degraded by ROS. The in vitro oxidative degradation rates of the PTK-URs followed first-order degradation kinetics, were significantly dependent on PTK composition (p<0.05), and displayed dose-dependence with respect to ROS levels. In subcutaneous rat wounds, PTK-UR scaffolds supported cellular infiltration and granulation tissue formation, followed first-order degradation kinetics over 7 weeks, and produced significantly greater stenting of subcutaneous wounds compared to PEUR scaffolds. These combined results indicate that PTK-UR tissue engineering scaffolds have significant advantages over analogous polyester-based biomaterials and provide a robust, cell-degradable substrate for guiding new tissue formation.
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