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Title page for ETD etd-11202015-152348

Type of Document Master's Thesis
Author Miller, Sinead Emily
Author's Email Address sinead.e.miller@vanderbilt.edu
URN etd-11202015-152348
Title Colistin-Functionalized Nanoparticles for the Rapid Capture of Acinetobacter baumannii
Degree Master of Science
Department Biomedical Engineering
Advisory Committee
Advisor Name Title
Todd D. Giorgio Committee Chair
Frederick R. Haselton Committee Member
  • Lipopolysaccharide
  • Gram-negative
  • Multi-Drug Resistance
  • Gold
Date of Defense 2015-11-19
Availability unrestricted
Gold nanoparticles (AuNPs) were functionalized for rapid binding of Acinetobacter baumannii (A. baumannii), a Gram-negative bacterium. AuNPs were functionalized with colistin (Col), a polycationic antibiotic, using a two-step self-assembly process, in which heterobifunctional polyethylene glycol (PEG) was used as a linker. Colistin was successfully conjugated to the AuNPs (Col-PEG-AuNP), as validated by dynamic light scattering (DLS) and proton nuclear magnetic resonance (H1 NMR). Images taken using a scanning transmission electron microscope (STEM), in combination with x-ray energy dispersive spectroscopy (EDS), confirmed binding of Col-PEG-AuNPs to the cell wall of A. baumannii. Results generated from a rapid binding assay indicated that the reaction rate of Col-PEG-AuNP complexation with A. baumannii reached half-maximum saturation in approximately 7 minutes. Quantitative measurement of the kinetics of Col-PEG-AuNP binding to A. baumannii is essential to inform the design of colistin-functionalized magnetic nanoparticles for magnetic separation of nanoparticle-bound A. baumannii.
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