A joint project of the Graduate School, Peabody College, and the Jean & Alexander Heard Library

Title page for ETD etd-11082006-102347

Type of Document Dissertation
Author Virostko, John Michael
Author's Email Address jack.virostko@vanderbilt.edu
URN etd-11082006-102347
Title In Vivo Imaging of the Islets of Langerhans
Degree PhD
Department Biomedical Engineering
Advisory Committee
Advisor Name Title
E. Duco Jansen Committee Chair
Alvin C Powers Committee Member
James M. Joers Committee Member
John C. Gore Committee Member
Michael I. Miga Committee Member
  • Islands of Langerhans -- Imaging
  • bioluminescence
  • islets
Date of Defense 2006-11-03
Availability unrestricted
The islets of Langerhans play a central role in maintenance of glucose homeostasis. Apoptosis of the insulin-producing beta cells of the islet leads to diabetes mellitus. The ability to non-invasively image or assess the islets of Langerhans would yield valuable insight into the progression of diabetes, guiding interventions intended to slow or halt diabetes progression and enabling assessment of islets after transplantation. The primary objective of this dissertation was to establish and validate a technique capable of non-invasively imaging the islet of Langerhans. The bulk of this work focuses on the use of bioluminescence imaging. Islets were engineered to express the luciferase optical reporter gene and applied to murine models of diabetes and transplant settings. In order to accurately quantify information from in vivo bioluminescence imaging, light-emitting standards were employed to determine sources of variance and standardize measurements. A three-dimensional bioluminescence reconstruction algorithm was characterized using these light-emitting standards and applied to bioluminescent islets. As bioluminescence imaging is limited to small animal models, clinically relevant islet imaging approaches using magnetic resonance imaging and target contrast agents were evaluated.
  Filename       Size       Approximate Download Time (Hours:Minutes:Seconds) 
 28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access 
  JMV_Dissertation_Final_ETD_Format.pdf 1.84 Mb 00:08:31 00:04:23 00:03:50 00:01:55 00:00:09

Browse All Available ETDs by ( Author | Department )

If you have more questions or technical problems, please Contact LITS.