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Title page for ETD etd-10282009-215831


Type of Document Dissertation
Author Huffman, Brian Joseph
URN etd-10282009-215831
Title Nanoscale Gold for Enhanced Protein Electrochemistry, Ebolavirus Immunosensors, and In Vivo Distribution Measurements
Degree PhD
Department Chemistry
Advisory Committee
Advisor Name Title
David E. Cliffel Committee Chair
David W. Wright Committee Member
Eva M. Harth Committee Member
Ray L. Mernaugh Committee Member
Keywords
  • Quart Crystal Microbalance
  • Toxicity
  • Ebola
  • TMPC
  • Nanoparticles
Date of Defense 2009-08-28
Availability unrestricted
Abstract
Different applications using gold at the nanoscale level are discussed in this dissertation. The first section includes relevant background into nanoparticles, specifically, monolayer protected gold clusters (MPC), and how they can be used in a myriad of applications. In the second and third section, a quartz crystal microbalance biosensor to detect ebolavirus and its glycoproteins is discussed. Included is an understanding instrument operation and how the biosensor’s gold surface is tailored to increase efficiency and potential reusability. The fourth section of the dissertation covers the covalent linking of cytochrome-c to gold MPCs, and delves into the electrochemical properties of the new bioconjugate. In the last chapter, in vivo experiments utilizing a mouse model explore gold MPCs as a possible platform for vaccine development or contrast agent. The aforementioned chapters show the versatility of gold at the nanoscale level as it was used to successfully detect ebolavirus, allow electron transfer between itself and a protein, and map distribution in vivo using a model system.
Files
  Filename       Size       Approximate Download Time (Hours:Minutes:Seconds) 
 
 28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access 
  AppendixACovalentBiosensor.pdf 413.49 Kb 00:01:54 00:00:59 00:00:51 00:00:25 00:00:02
  AppendixBAdditionalHistology.pdf 161.50 Kb 00:00:44 00:00:23 00:00:20 00:00:10 < 00:00:01
  Chapter1NanoparticleIntro.pdf 496.30 Kb 00:02:17 00:01:10 00:01:02 00:00:31 00:00:02
  Chapter2EbolaGPandQCMbackground.pdf 1.38 Mb 00:06:23 00:03:17 00:02:52 00:01:26 00:00:07
  Chapter3WholeEbolaDetector.pdf 300.18 Kb 00:01:23 00:00:42 00:00:37 00:00:18 00:00:01
  Chapter4Cytc.pdf 665.73 Kb 00:03:04 00:01:35 00:01:23 00:00:41 00:00:03
  Chapter5TMPCsinMice.pdf 830.55 Kb 00:03:50 00:01:58 00:01:43 00:00:51 00:00:04
  dedication.pdf 8.25 Kb 00:00:02 00:00:01 00:00:01 < 00:00:01 < 00:00:01
  ElectronicTITLEPAGE.pdf 4.54 Kb 00:00:01 < 00:00:01 < 00:00:01 < 00:00:01 < 00:00:01
  ListofFigures.pdf 41.73 Kb 00:00:11 00:00:05 00:00:05 00:00:02 < 00:00:01
  ListofTables.pdf 3.54 Kb < 00:00:01 < 00:00:01 < 00:00:01 < 00:00:01 < 00:00:01
  TableofContents.pdf 8.50 Kb 00:00:02 00:00:01 00:00:01 < 00:00:01 < 00:00:01

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