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Title page for ETD etd-07282009-114232


Type of Document Dissertation
Author Harris, Mark Philip
Author's Email Address mark.harris@vanderbilt.edu
URN etd-07282009-114232
Title The Study of Cancer Cell Heterogeneity, by Image-based Migration Analysis
Degree PhD
Department Cancer Biology
Advisory Committee
Advisor Name Title
Donna Webb Committee Chair
Alissa Weaver Committee Member
Jeffery Davidson Committee Member
Vito Quaranta Committee Member
Yu Shyr Committee Member
Keywords
  • single-cell
  • dynamic
  • multivariate
  • variability
  • heterogeneity
Date of Defense 2009-07-13
Availability unrestricted
Abstract
Genetic heterogeneity is a key factor involved in tumor progression, and it is increasingly recognized that heterogeneity of phenotypic traits exists among individual cancer cells. In this dissertation, I investigate the heterogeneity of cancer cell motility via video-microscopic analysis by: 1) quantitating heterogeneity of cell motility, 2) exploring the differences in cell motility between non-tumorigenic and cancer cell lines, and 3) developing novel tools and metrics for the study of dynamic characteristics of cancer cells. To this end, over 7,300 cells were tracked and analyzed, from a variety of cell lines, in an assortment of microenvironmental conditions. The work presented in this dissertation provides important additions to the study of single-cell variability, and provides robust tools for future investigations in this field.
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