| Type of Document |
Dissertation |
| Author |
Long, Amanda Ashleigh
|
| URN |
etd-07262010-130933 |
| Title |
Characterization of novel genes regulating the
synaptic vesicle cycle in drosophila
|
| Degree |
PhD |
| Department |
Biological Sciences |
| Advisory Committee |
| Advisor Name |
Title |
| Todd Graham |
Committee Chair |
| David Miller |
Committee Member |
| Donna Webb |
Committee Member |
| Kendal Broadie |
Committee Member |
|
| Keywords |
- photoreceptor
- synapse
- neurotransmission
- synaptic vesicle cycle
- Drosophila
- mRNA regulation
- Smg1
- NMD pathway
|
| Date of Defense |
2010-07-12 |
| Availability |
unrestricted |
Abstract
A systematic Drosophila forward genetic screen for photoreceptor synaptic transmission mutants identified no-on-and-no-off transient C (nonC) based on loss of retinal synaptic responses to light stimulation. The cloned gene encodes phosphatidylinositol 3-kinase-like kinase (PIKK) Smg1, a regulatory kinase of the nonsense-mediated decay (NMD) pathway. The Smg proteins act in an mRNA quality control surveillance mechanism to selectively degrade transcripts containing premature stop codons, thereby preventing the translation of truncated proteins with dominant negative or deleterious gain of function activities. At the neuromuscular junction (NMJ) synapse, an extended allelic series of Smg1 mutants show impaired structural architecture, with decreased terminal arbor size, branching and synaptic bouton number. Functionally, loss of Smg1 results in a ~50% reduction in basal neurotransmission strength, as well as progressive transmission fatigue and greatly impaired synaptic vesicle recycling during high-frequency stimulation. Mutation of other NMD pathways genes (Upf2 and Smg6) similarly impairs neurotransmission and synaptic vesicle cycling. These findings suggest that the NMD pathway acts to regulate proper mRNA translation to safeguard synapse morphology and maintain synaptic functional efficacy.
|
| Files |
| Filename |
Size |
Approximate Download Time
(Hours:Minutes:Seconds)
|
| 28.8 Modem |
56K Modem |
ISDN (64 Kb) |
ISDN (128 Kb) |
Higher-speed Access |
| |
ALThesis.pdf |
4.06 Mb |
00:18:46 |
00:09:39 |
00:08:27 |
00:04:13 |
00:00:21 |
|
