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Title page for ETD etd-07132009-114737


Type of Document Dissertation
Author Roberts, Randolph K.
URN etd-07132009-114737
Title Analysis of signaling pathways important in the specification and migration of oligodendrocyte progenitor cells in the zebrafish spinal cord
Degree PhD
Department Biological Sciences
Advisory Committee
Advisor Name Title
Lilianna Solnica-Krezel Committee Chair
Bruce Appel Committee Member
Chris Janetopoulos Committee Member
E. Michelle Southard-Smith Committee Member
Joshua Gamse Committee Member
Keywords
  • cell polarity
  • stem cells
  • zebrafish
  • CNS
Date of Defense 2009-05-15
Availability unrestricted
Abstract
During spinal cord development, precursor cells transition from proliferative divisions to differentiative divisions. Traditionally proliferative divisions, which increase cell numbers, are thought to be symmetric, whereas differentiative divisions are thought to occur both by symmetric and asymmetric divisions. Currently, the mechanisms that control this differentiative cell division fate remain to be defined. However, studies conducted using atypical protein kinase C (aPKC) suggests that aPKC has a conserved function in controlling cell division orientation. In this study, we look at the role aPKC may play in maintaining precursor division in the zebrafish spinal cord. Through time-lapse imaging and loss of function studies we were able to show that aPKC does regulate precursor division in the zebrafish spinal cord, and in its absence excess oligodendrocyte precursor cells (OPCs) are specified at the possible expense of adult precursors.
Files
  Filename       Size       Approximate Download Time (Hours:Minutes:Seconds) 
 
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  FullThesis.pdf 2.71 Mb 00:12:33 00:06:27 00:05:39 00:02:49 00:00:14
  IntroThesisPages.pdf 65.27 Kb 00:00:18 00:00:09 00:00:08 00:00:04 < 00:00:01

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