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Title page for ETD etd-06302008-153406


Type of Document Dissertation
Author Misra, Sunita N
Author's Email Address sunita.misra@vanderbilt.edu
URN etd-06302008-153406
Title Characterization of Mutant Human Brain Sodium Channels Associated with Familial Epilepsy
Degree PhD
Department Pharmacology
Advisory Committee
Advisor Name Title
P. Jeffrey Conn Committee Chair
Alfred L. George, Jr. Committee Member
Danny G. Winder Committee Member
Gregory Mathews Committee Member
Robert L. Macdonald Committee Member
Vsevolod V. Gurevich Committee Member
Keywords
  • Sodium channels -- Pathophysiology
  • electrophysiology
  • epilepsy
  • Epilepsy -- Genetic aspects
  • Epilepsy -- Molecular aspects
Date of Defense 2008-06-09
Availability unrestricted
Abstract
Investigating genetic forms of epilepsy allows for improved understanding of epilepsy pathophysiology in general. Mutations in voltage-gated sodium channels are a frequent cause of genetic forms of epilepsy. First we constructed a computational model of one sodium channel isoform and an epilepsy-associated mutation that refined our knowledge of how sodium channels inactivate. We next utilized a heterologous expression system to biophysically and biochemically characterize epilepsy-associated mutations. We found that aberrant cell surface expression as well as biophysical abnormalities may underlie some epilepsy syndromes. Finally we performed experiments in a transgenic mouse model system of epilepsy to examine the effect of genetic modifiers on sodium channel function. In summary, this research utilized three different model systems to improve our understanding of genetic forms of epilepsy.
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