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Title page for ETD etd-06182014-172653


Type of Document Dissertation
Author Grimaldi, Ashley Dyan
URN etd-06182014-172653
Title A Role for CLASPs in the Regulation of the Major Microtubule +TIP Complex Organizers, End-Binding Proteins
Degree PhD
Department Cell and Developmental Biology
Advisory Committee
Advisor Name Title
Laura Lee Committee Chair
Matthew Lang Committee Member
Matthew Tyska Committee Member
Ryoma Ohi Committee Member
Keywords
  • MTs
  • CLASPs
  • Microtubule
  • EBs
  • +TIP Complex
  • End-Binding
Date of Defense 2014-06-16
Availability unrestricted
Abstract
Microtubule plus-end tracking proteins (+TIPs) are a specialized group of microtubule-associated proteins (MAPs) that preferentially localize to microtubule (MT) plus-ends. This unique localization is thought to be due to +TIP recognition of a unique structural or chemical feature found only at the plus-ends of MTs, such as the GTP- tubulin cap. End-binding proteins (EBs) are considered the master regulators of the +TIP network; however, whether EBs are regulated by other +TIP proteins is not well understood. Therefore, a study of the regulation of EB proteins by CLASPs (CLIP-associated proteins), another family of +TIPs, was undertaken.

In cells lacking CLASPs, EB binding at MTs is significantly altered: both EB1 and EB3 relocalized to the MT lattice in addition to their characteristic plus-end localization. This study reveals that CLASPs likely function during MT polymerization to support efficient GTP-hydrolysis and thus promote specific EB plus-end localization. This work establishes CLASPs as crucial determinants of proper EB localization at MTs in cells.

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