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Title page for ETD etd-06032009-182327


Type of Document Dissertation
Author Orndorff, Rebecca Lee
URN etd-06032009-182327
Title High Affinity Peptide Neurotoxin Quantum Dot Conjugates for Detecting Endogenous Targets in Live Cells and Ex Vivo Tissue
Degree PhD
Department Chemistry
Advisory Committee
Advisor Name Title
Sandra J. Rosenthal, Ph.D. Committee Chair
David W. Wright, Ph.D. Committee Member
Eva M. Harth, Ph.D. Committee Member
Michael P. Stone, Ph.D. Committee Member
Keywords
  • matrix metalloproteinase-2
  • glioma
  • cancer
  • neuromuscular junction
  • biotechnology
  • MMP-2
  • cys-loop
  • potassium channels
  • voltage-gated potassium channel
  • Kv1.1
  • ligand-gated ion channel
Date of Defense 2009-05-29
Availability unrestricted
Abstract
This project focused upon advancing nanotechnology for applications in biological assays using highly fluorescent nanocrystals, quantum dots, with high affinity peptide neurotoxins as targeting molecules. Specifically, this work utilized three high affinity peptide neurotoxins, alpha-bungarotoxin, chlorotoxin, and dendrotoxin-1, as ligands to facilitate quantum dot detection of their respective biological targets. Two different quantum dot detection methodologies—direct ligand conjugation to the quantum dot surface, and exploitation of streptavidin quantum dot affinity for biotinylated ligands—were employed to label the endogenously expressed proteins. These methods were demonstrated to effectively detect endogenous cellular proteins in a living environment, and to label nicotinic acetylcholine receptors in mouse diaphragm tissue ex vivo. The results of this research effort support high affinity peptide neurotoxin quantum dot conjugate integration into biological assays.
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