A joint project of the Graduate School, Peabody College, and the Jean & Alexander Heard Library

Title page for ETD etd-05182016-161820


Type of Document Dissertation
Author Clay, Hayley Boyd
URN etd-05182016-161820
Title Roles of the mitochondrial fatty acid synthesis II (mtFASII) pathway in mitochondrial function and signaling
Degree PhD
Department Neuroscience
Advisory Committee
Advisor Name Title
Aaron Bowman Committee Chair
Bruce Carter Committee Member
Deborah Murdock Committee Member
Laura Dugan Committee Member
Sandra Zinkel Committee Member
Keywords
  • mitochondrial signaling
  • metabolomics
Date of Defense 2016-05-09
Availability restricted
Abstract
Despite the presence of a cytosolic fatty acid synthesis pathway, mitochondria have retained their own means of creating fatty acids via the mitochondrial fatty acid synthesis (mtFASII) pathway. The reason for its conservation has not yet been elucidated. Therefore, to better understand the role of mtFASII in the cell, we used a variety of methods to characterize the consequences of changes in mtFASII functionality in whole cells, isolated mitochondria, and mitochondrial secretions. We altered mtFASII functionality by knockdown of acyl carrier protein (ACP) or overexpression of mitochondrial trans-2-enoyl-CoA reductase (MECR). As a control for known respiratory deficits in mtFASII knockdowns, we also knocked down a component of complex I of the electron transport chain. We found that loss of mtFASII function disturbs metabolism and bioactive lipid regulation at the whole-cell and mitochondrial levels. We found that the mitochondrial secretome may contain bioactive lipids and small peptides, and that knockdown of the mtFASII pathway results in increased levels of dipeptides, among other metabolites, in the mitochondrial secretome. These data indicate that the mtFASII pathway may have a role in mitochondrial signaling in a manner not linked to mtFASII’s effects on the electron transport chain.
Files
  Filename       Size       Approximate Download Time (Hours:Minutes:Seconds) 
 
 28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access 
[campus] Clay.pdf 3.99 Mb 00:18:27 00:09:29 00:08:18 00:04:09 00:00:21
[campus] indicates that a file or directory is accessible from the campus network only.

Browse All Available ETDs by ( Author | Department )

If you have more questions or technical problems, please Contact LITS.