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Title page for ETD etd-04182013-154535


Type of Document Dissertation
Author Broderick, Sarah M
URN etd-04182013-154535
Title Genetic Analysis of Ninjurin A, A stress-regulated potein that induces nonapoptotic cell death
Degree PhD
Department Cell and Developmental Biology
Advisory Committee
Advisor Name Title
David Miller Committee Chair
Andrea Page-McCaw Committee Member
Ethan Lee Committee Member
Heather Broihier Committee Member
Julian Hillyer Committee Member
Laura Lee Committee Member
Keywords
  • Cell Biology
Date of Defense 2012-07-09
Availability unrestricted
Abstract
Ninjurins are a conserved family of transmembrane proteins that increase expression in response to injury and stress. There are few in vivo studies of Ninjurin, and little is known about Ninjurin function. In this thesis I investigated the immune function of a recently generated null mutant of NijA, which did not display a detectable phenotype. Expression studies of the NijA protein show redistribution to the cell surface in larval immune tissues after septic injury. The NijA protein is also upregulated by constitutive activation of the Toll pathway. Ectopic expression of NijA induced cell death. These dying cells appeared to die by nonapoptotic cell death because they did not display hallmarks of apoptotic cells including TUNEL staining and inhibition by p35. Preliminary studies investigating UV-irradiation induced cell death in the NijA null mutant suggests that NijA maybe required to induce stress-activated cell death. These results suggest a role for NijA in stress-activated nonapoptoic cell death.
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