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Title page for ETD etd-04132010-093536


Type of Document Master's Thesis
Author Makowski, Alexander James
URN etd-04132010-093536
Title In Vivo Raman Spectroscopic Analysis of HSP70 and Laser Preconditioning in Murine Incisional Wounds
Degree Master of Science
Department Biomedical Engineering
Advisory Committee
Advisor Name Title
E. Duco Jansen Committee Chair
Anita Mahadevan-Jansen Committee Member
Keywords
  • spectroscopy
  • protein folding
  • cellularity
  • peak ratio
  • Raman
  • HSP 70
  • wound healing
  • preconditioning
  • laser
Date of Defense 2010-04-11
Availability unrestricted
Abstract
Laser preconditioning augments incisional wound healing by reducing scar tissue and increasing maximum tensile load of the healed wound. Under the hypothesis that HSP70 plays an active role in reported results and to better understand the downstream effects of laser preconditioning, this study utilized a probe-based Raman spectroscopy system to achieve an in-vivo, spatio-temporal biochemical profile of murine skin incisional wounds as a function of laser preconditioning and the presence of HSP70. Raman spectra yielded significant differences in known biochemical peaks between wild-type (WT) and HSP70 knockout mice. Analysis of peak ratios indicated 1) an increase in protein configuration on and surrounding the wound, and 2) increased cellularity that was prolonged in WT mice due to laser treatment. HSP70 is active in protein configuration and cellularity of early wound healing. Laser preconditioning enhances the effects of HSP70 in ways consistent with findings of previous studies. Raman spectroscopy proved a convenient non-invasive method of obtaining information for evaluating these effects and efficacy of wound healing laser treatment.
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