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Title page for ETD etd-04022012-134344


Type of Document Master's Thesis
Author Hughes, Emma Bronwyn
Author's Email Address emma.b.hughes@vanderbilt.edu
URN etd-04022012-134344
Title High affinity ligands for the targeting of SERT and NET
Degree Master of Science
Department Chemistry
Advisory Committee
Advisor Name Title
Dr. Sandra Rosenthal Committee Chair
Dr. David Wright Committee Member
Dr. Eva Harth Committee Member
Keywords
  • quantum dots
  • norepinephrine transporter
  • serotonin transporter
  • single molecule tracking
  • mental disorders
Date of Defense 2012-04-02
Availability unrestricted
Abstract

Mental health problems related to dysfunction of serotonin transporter protein and norepinephrine transporter protein are an ever increasing burden on the population and the health care system. The Rosenthal lab uses a unique system that employs small molecule targeting ligands and quantum dots to image SERT and NET in live cells. The movement of the transporter proteins can be imaged in real time for much longer than with traditional flurophores due to the unique properties of the quantum dots. This project focused on testing new targeting ligands. The SERT targeting ligand IDT361 showed the ability to label SERT with quantum dots in live Hek293T cells. NET targeting ligand IDT545 was tested in live cells but did show high enough specificity to deter the uptake ability of NET in live cells and therefore is unlikely to be used in further study.

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