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Title page for ETD etd-04022007-172202


Type of Document Dissertation
Author Smith, Travis Kirk
URN etd-04022007-172202
Title Analysis of Bves function through identification of interacting proteins.
Degree PhD
Department Cell and Developmental Biology
Advisory Committee
Advisor Name Title
Chris Wright Committee Chair
David Bader Committee Member
Roland Stein Committee Member
Scott Baldwin Committee Member
Tsutomu Kume Committee Member
Keywords
  • Cdc42
  • Rac1
  • Rho GTPases
  • antibodies
  • cell motility
  • Bves
  • GEFT
Date of Defense 2006-12-19
Availability unrestricted
Abstract
The work contained in this document describes the generation of new immunoreagents for Bves study, and our identification of a Bves-interacting protein. After generating new monoclonal antibodies to the Bves protein, I here describe the characterization of Bves expression throughout mouse embryogenesis, clearly demonstrating that Bves is widely expressed in many tissue types throughout gestation. I also describe the isolation of Geft as a directly interacting protein for Bves, and biochemically confirm and characterize the interaction between these two proteins. I then demonstrate a role for Bves in control of cellular motility via modulation of the Rac1/Cdc42 GTPases, likely through the Geft interaction. These findings represent both the first identification of a Bves-interacting protein and the first placement of Bves into a molecular pathway within the cell. The data presented here will undoubtedly serve as a departure point for many future investigations into the Bves protein, and represent seminal findings in the study of Bves function.
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