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Title page for ETD etd-03312008-114140


Type of Document Master's Thesis
Author Cayce, Jonathan Matthew
Author's Email Address jonathan.m.cayce@vanderbilt.edu
URN etd-03312008-114140
Title Infrared neural stimulation of thalamocortical brain slices in vitro
Degree Master of Science
Department Biomedical Engineering
Advisory Committee
Advisor Name Title
Anita Mahadevan-Jansen Committee Chair
E. Duco Jansen Committee Member
Keywords
  • laser
  • infrared neural stimulation
  • neural stimulation techniques
  • brain slice
  • somatosensory
Date of Defense 2008-03-31
Availability unrestricted
Abstract
Neural stimulation using infrared light has recently been characterized as a novel method to stimulate peripheral nerves without touching, causing damage, or inducing an electrical stimulation artifact. Infrared neural stimulation (INS) has not been previously achieved in the brain due to the complexity of the neuronal networks. The purpose of this study was to show feasibility of INS in the central nervous system, and determine the optimal parameters for INS in a thalamocortical brain slice model. Wavelength was the first parameter identified since previous studies showed penetration depth of light in tissue determined the threshold radiant energies needed to evoke an action potential in the peripheral nervous system. The wavelength of 3.65 &181m was determined to be the optimal wavelength. Next repetition rate was investigated using the optimal wavelength of 3.65 &181m. Lower threshold radiant energies were observed for higher repetition rates. The final parameter investigated was spot size using light at 3.65 &181m and 30 Hz, and a third order power fit relationship was observed where a larger spot size required less energy to evoke action potentials in a TC slice. A small set of experiments were performed to show intracellular electrical recordings could be used to detect INS evoked signals. The results from this study prove feasibility of INS in CNS, and provide the basis for future in vivo experiments.
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