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Title page for ETD etd-03302007-130416


Type of Document Dissertation
Author Maginnis, Melissa Sue
Author's Email Address melissa.maginnis@vanderbilt.edu
URN etd-03302007-130416
Title Identification and characterization of cellular determinants of reovirus internalization.
Degree PhD
Department Microbiology and Immunology
Advisory Committee
Advisor Name Title
Luc Van Kaer Committee Chair
Mary M. Zutter Committee Member
Paul Spearman Committee Member
Roy Zent Committee Member
Terence S. Dermody Committee Member
Keywords
  • endocytosis
  • nonenveloped virus
  • virus receptor
  • beta1 integrin
  • JAM-A
  • NPXY motif
  • Reoviruses
  • Host-virus relationships
Date of Defense 2007-03-23
Availability unrestricted
Abstract
Virus cell entry is a multi-step process that often requires independent receptors for attachment and internalization. Reovirus infection is initiated by attachment to cell-surface carbohydrate and junctional adhesion molecule-A. Findings reported in this thesis indicate that following attachment to JAM-A, Beta1 integrin facilitates reovirus internalization into target cells. Importantly, tyrosine-based signaling motifs in the Beta1 integrin cytoplasmic tail are required for transport of reovirus to the appropriate endocytic organelle for viral disassembly. Together, these data provide evidence that Beta1 integrin facilitates reovirus internalization and suggest that viral entry occurs by interactions of reovirus virions with independent attachment and entry receptors on the cell surface. These results give new insights into mechanisms of reovirus internalization and functions of Beta1 integrin in endocytosis.
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