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Title page for ETD etd-03282011-140349


Type of Document Dissertation
Author Kerr, Thomas Joshua
Author's Email Address josh.kerr@vanderbilt.edu
URN etd-03282011-140349
Title Development of Novel Ion Mobility-Mass Spectrometry Shift Reagents for Proteomic Applications
Degree PhD
Department Chemistry
Advisory Committee
Advisor Name Title
Prof. John McLean Committee Chair
Prof. Chris Janetopoulos Committee Member
Prof. David Cliffel Committee Member
Prof. Eva Harth Committee Member
Keywords
  • mass spectrometry
  • ion mobility
  • proteomics
Date of Defense 2011-03-11
Availability unrestricted
Abstract
Novel ion mobility-mass spectrometry shift reagents have been developed in order to facilitate rapid identification of specific functionalities within a complex mixture of peptides. Lanthanide chelating labels provide great flexibility and can be used for relative quantitation experiments, as well as IM-MS shift reagents when analyzed using ion mobility instrumentation. Evaluation of optimum labeling strategies and limitations were explored and complex samples were analyzed for the application of these shift reagents to analysis of complex mixtures of peptides. Furthermore, progress toward developing shift reagents for use in labeling other functionalities is briefly outlined.
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