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Title page for ETD etd-03262013-155906


Type of Document Dissertation
Author Yufenyuy, Ernest Limnyuy
URN etd-03262013-155906
Title The Role Of Intersubunit Interfaces In HIV-1 Capsid Assembly And Stability
Degree PhD
Department Microbiology and Immunology
Advisory Committee
Advisor Name Title
Earl Ruley Committee Chair
Ben Spiller Committee Member
Chris Aiken Committee Member
Ellen Fanning Committee Member
Mark Denison Committee Member
Keywords
  • Assembly
  • HIV-1
  • Uncoating
  • Capsid
Date of Defense 2013-03-20
Availability unrestricted
Abstract
This dissertation discusses the role of intersubunit interfaces in HIV-1 capsid assembly and stability. In this study I showed the presence of both the NTD-CTD and CTD-CTD trimer interfaces in mature HIV-1 virions using engineered disulfide crosslinking. Using mutagenesis, I showed that these interfaces are critically important for assembly and stability of the HIV-1 capsid. I also showed that the CTD-CTD trimer interface is formed only after cleavage of CA-SP1. My results provide valuable fundamental knowledge about the biology of capsid assembly and stability and highlight the viral capsid as an attractive target for anti-HIV therapy.
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