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Title page for ETD etd-03262013-104441


Type of Document Dissertation
Author Brucker, Robert Merrill
Author's Email Address bruckerm@gmail.com
URN etd-03262013-104441
Title Speciation microbiomes: consequences of gut bacteria on hybrid mortality in the genus Nasonia
Degree PhD
Department Biological Sciences
Advisory Committee
Advisor Name Title
Patrick Abbot Committee Chair
Antonis Rokas Committee Member
Eric Skaar Committee Member
Juergen Gadau Committee Member
Seth Bordenstein Committee Member
Keywords
  • innate immunity
  • hologenome
  • parasitoid
  • hybrid mortality
  • phylosymbiosis
  • symbiosis
  • Nasonia
  • Evolution
  • microbiota
  • microbiome
Date of Defense 2013-03-19
Availability unrestricted
Abstract
Symbiosis is a process by which two or more distinct organisms interact, whereas speciation is the diversifying process by which one species splits into two. Symbiosis and speciation are not commonly discussed together and can seem to be odd partners in their capacity to operate synergistically in nature. However, the complex community of microorganisms that live in symbiosis within an animal species—known as the microbiome—has the capacity to confer new traits and selective pressures that drive speciation. To demonstrate the microbiome’s influence in speciation, we present the following evidence using closely related insect species of the model Nasonia. First, the gut microbiome forms phylosymbiotic assemblages in which the relationships of the Nasonia-associated microbiomes parallel the evolutionary genetic relationships of Nasonia. Second, these phylosymbiotic assemblages become irregular in hybrids between the species. Third, the altered microbiome in hybrids is correlated with high rates of hybrid mortality. Fourth, when the hybrids are cured of their irregular microbiota, they are rescued from hybrid mortality. Finally, when the cured hybrids were reinoculated with bacteria, they recapitulated high rates of mortality. We conclude that in this animal complex, the gut microbiome is equally important as host genes in promoting hybrid mortality and thus advancing speciation.
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