A joint project of the Graduate School, Peabody College, and the Jean & Alexander Heard Library

Title page for ETD etd-03252014-155409


Type of Document Master's Thesis
Author Schroer, Alison Koelle
Author's Email Address alison.k.schroer@vanderbilt.edu
URN etd-03252014-155409
Title Integrin and cytokine signaling in myofibroblast differentiation: a network modeling approach
Degree Master of Science
Department Biomedical Engineering
Advisory Committee
Advisor Name Title
W. David Merryman Committee Chair
John P. Wikswo Committee Member
Keywords
  • focal adhesion kinase (FAK)
  • Src
  • fibroblast growth factor (FGF)
  • transforming growth factor beta (TGF-β1)
  • myofibroblast differentiation
  • Alpha-smooth muscle actin (αSMA)
  • p38
  • extracellular signaling-related kinase (ERK)
  • integrins
Date of Defense 2014-03-25
Availability unrestricted
Abstract
Fibrotic disease is a major cause of morbidity and mortality and is characterized by the transition of resident fibroblast cells into active myofibroblasts, identified by their expression of alpha smooth muscle actin. Myofibroblast differentiation is regulated by growth factor signaling and mechanical signals transduced through integrins, which converge at focal adhesion proteins (Src and FAK) and MAPK signaling, but lead to divergent outcomes. While details are known about individual pathways, little is known about their interactions. To this end, an ODE-based model of this cell signaling network was developed in parallel with in vitro experiments to analyze potential mechanisms of crosstalk and regulation of αSMA production. We found that cells lacking Src or FAK produce significantly less or more αSMA than wild type cells, respectively. Transforming growth factor beta 1 and fibroblast growth factor signal through ERK and MAPK p38 with different dynamic profiles to increase or decrease αSMA expression, respectively. Our model effectively recreated αSMA expression levels across a set of 22 experimental conditions and matched transient phosphorylation of ERK and p38. These results support a potential mechanism for regulation of fibroblast differentiation: αSMA production is promoted by active p38 and Src and opposed by ERK.
Files
  Filename       Size       Approximate Download Time (Hours:Minutes:Seconds) 
 
 28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access 
  ASchroer.pdf 1.28 Mb 00:05:54 00:03:02 00:02:39 00:01:19 00:00:06

Browse All Available ETDs by ( Author | Department )

If you have more questions or technical problems, please Contact LITS.