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Title page for ETD etd-03242010-175917


Type of Document Master's Thesis
Author Nathaniel, Elizabeth Anne Nalani
URN etd-03242010-175917
Title Characterization of Band 3 Variant G130R Using Site-Directed Spin Labeling and Electron Paramagnetic Resonance
Degree Master of Science
Department Chemical and Physical Biology
Advisory Committee
Advisor Name Title
Charles Cobb Committee Chair
Al Beth Committee Member
Hassane Mchaourab Committee Member
Keywords
  • erythrocyte cytoskeleton
  • anion exchanger 1
  • CW-EPR
  • power saturation
Date of Defense 2010-03-01
Availability unrestricted
Abstract
In this thesis, the structural differences between wild-type band 3 and band 3 Fukuoka, the G130R mutant identified in cases of hereditary spherocytosis associated with reduction of protein 4.2, are examined. The G130R mutation occurs on the solvent-facing side of helix 2 on the globular portion of the cytosolic domain of band 3. Site-directed spin labeling coupled with electron paramagnetic resonance techniques are presented as methods for investigating subtle changes to protein structure. While the overall structure of the band 3 dimer shows little change, side chain mobilities are altered on helix 2. Solvent accessibility, on the other hand, shows that helix 2 maintains its backbone structure and relative orientation on the surface of band 3. I believe these small changes to helix 2 affect the binding of band 3 to ankyrin due to the proximity to their interaction site.
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