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Title page for ETD etd-02282017-115225

Type of Document Dissertation
Author Moore, Jessica Lindsey
Author's Email Address jessica.lindsey.moore@gmail.com
URN etd-02282017-115225
Title Imaging Mass Spectrometry and Microbial Pathogenesis: The Effect of Calprotectin on Pathogen-Host Interactions
Degree PhD
Department Chemistry
Advisory Committee
Advisor Name Title
Richard Caprioli Committee Chair
David Cliffel Committee Member
Eric Skaar Committee Member
John McLean Committee Member
Walter Chazin Committee Member
  • Imaging Mass Spectrometry
  • S aureus
  • Calprotectin
  • proteomics
  • post-translational modifications
  • pathogen-host interactions
Date of Defense 2017-02-13
Availability restricted
Disease is characterized by signature molecular changes in affected tissues and organs. Diseases cause disruption and dysregulation of a number of biological molecules, including proteins, lipids, metal, and small molecules. Understanding the spatial distribution of biomolecules as it relates to human disease is incredibly important; it represents a way to study disease-associated changes in tissues. This work utilizes Imaging Mass Spectrometry (IMS), a discovery-based analytical approach that enables the detection of biological molecules spatially within diseased tissues, to study infectious diseases. IMS discovered molecular changes specific to areas in the tissue where pathogens interact with their vertebrate hosts. This region, deemed the pathogen-host interface, presents a wealth of information about how vertebrate hosts defend themselves from invading pathogens, including the accumulation and oxidative damage of the metal-chelating host protein calprotectin. In response to metal-starvation, bacteria also exhibit characteristic changes that are observable by mass spectrometry. Further study of these interactions is paramount to the understanding of microbial pathogenesis and to the continued treatment of infectious diseases.
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