A joint project of the Graduate School, Peabody College, and the Jean & Alexander Heard Library

Title page for ETD etd-02252014-165554


Type of Document Dissertation
Author Fites, Jeffrey Scott
URN etd-02252014-165554
Title Evasion of adaptive immune defenses by the lethal chytrid fungus Batrachochytrium dendrobatidis
Degree PhD
Department Biological Sciences
Advisory Committee
Advisor Name Title
Katherine Friedman Committee Chair
Clint Carter Committee Member
D. Borden Lacy Committee Member
Julian Hillyer Committee Member
Louise Rollins-Smith Committee Member
Keywords
  • immunomodulation
  • fungal pathogenesis
  • amphibian immunology
  • Batrachochytrium dendrobatidis
  • chytridiomycosis
  • amphibian declines
Date of Defense 2014-02-14
Availability unrestricted
Abstract
In the sixth mass extinction, amphibians are being lost in greater numbers than almost any other taxon. The deadly skin disease, chytridiomycosis, has taken a great toll on amphibian populations around the globe. Batrachochytrium dendrobatidis, the causative agent of chytridiomycosis, appears to have evolved as an obligate pathogen and thus must evade destruction by the amphibian defenses in order to reproduce. Several defenses along the skin mucosa likely inhibit the initial colonization by B. dendrobatidis zoospores, but B. dendrobatidis persists longer in the epithelium where it must survive any onslaught by innate and adaptive immune responses. Batrachochytrium dendrobatidis does this by evading phagocytes inside keratinocytes and impairing lymphocytes that might coordinate a robust immune response. Soluble molecules produced by B. dendrobatidis inhibited B and T cell proliferation and induced lymphocyte apoptosis. These molecules were only produced by cell-walled life-cycle stages that are present in host tissue. Inhibiting cell-wall biosynthesis with a chitin-synthase inhibitor greatly reduced its capacity to inhibit lymphocytes, suggesting that inhibitory factors are located in the cell wall. Treatments of inhibitory factors showed that the soluble molecules are not likely to be proteins or common structural components found in fungal cell walls. Although the B. dendrobatidis cell wall has not been characterized, fungal cell walls are largely composed of carbohydrate suggesting that inhibitory factors may be small molecules and/or carbohydrates. These data suggest that B. dendrobatidis has evolved a survival strategy targeting adaptive immune defenses in the skin of amphibian hosts.
Files
  Filename       Size       Approximate Download Time (Hours:Minutes:Seconds) 
 
 28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access 
  FitesPhDThesis.pdf 4.29 Mb 00:19:52 00:10:13 00:08:56 00:04:28 00:00:22

Browse All Available ETDs by ( Author | Department )

If you have more questions or technical problems, please Contact LITS.