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Title page for ETD etd-01232009-152329


Type of Document Master's Thesis
Author Moore, Jessica Lauren
URN etd-01232009-152329
Title Amphetamine induces accumulation of the norepinephrine transporter into Rab4- and Rab11-positive compartments
Degree Master of Science
Department Interdisciplinary Studies: Membrane Transporter Biology
Advisory Committee
Advisor Name Title
Aurelio Galli Committee Co-Chair
Roger Colbran Committee Co-Chair
Keywords
  • Endosomes
  • endocytic recycling
  • Noradrenaline
  • Amphetamines -- Physiological effect
  • Carrier proteins -- Molecular aspects
Date of Defense 2009-01-23
Availability unrestricted
Abstract
The norepinephrine transporter (NET) clears norepinephrine (NE) from the synapse after vesicular release. NET is a target of the psychostimulant amphetamine (AMPH). We have recently shown that AMPH alters trafficking of the transporter, causing a net decrease in surface NET in the monoaminergic CAD cell line. In this study we demonstrate, by confocal imaging of immunostained superior cervical ganglion (SCG) neurons, that AMPH causes an increase in NET levels inside terminal boutons. Further, the intracellular compartment in which NET accumulates upon AMPH exposure is not known; such information would inform investigation of the mechanism by which the drug affects NET’s cellular distribution. We show that after AMPH treatment, NET co-fractionates with both Rab4 and Rab11, and that AMPH increases co-localization of NET with these endosomal markers as indicated by the intensity correlation quotient (ICQ). Finally, we show that the functions of both GTPases are involved in AMPH-regulated NET trafficking by transfection of dominant negative (DN) constructs followed by cell-surface biotinylation. Our results support the conclusion that AMPH-regulated NET trafficking occurs through endosomes.
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