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Title page for ETD etd-01142015-173558


Type of Document Dissertation
Author Spencer, Cierra Tamese
URN etd-01142015-173558
Title Biochemical characterization of a Pseudomonas aeruginosa phospholipase D
Degree PhD
Department Pharmacology
Advisory Committee
Advisor Name Title
Dr. Tina Iverson Committee Chair
Dr. Craig Lindsley Committee Member
Dr. Eric Skaar Committee Member
Dr. H. Alex Brown Committee Member
Dr. Lawrence Marnett Committee Member
Keywords
  • phospholipase D
  • enzymology
  • bacterial virulence
Date of Defense 2014-12-30
Availability unrestricted
Abstract
Phospholipase D (PLD) is a ubiquitous enzyme found in prokaryotic and eukaryotic organisms that generates phosphatidic acid. A number of human bacterial pathogens produce PLD as virulence factors to promote infections. PldA of P. aeruginosa is one such example and not only modulates host cell signaling to promote cell invasion, but also mediates bacterial killing by perturbing cell wall stability. Both of these processes may directly and indirectly contribute to host colonization by P. aeruginosa. Despite the recognized significant of these bacterial PLD, little progress has been made toward understand their enzymatic activity in vitro or upon interaction with host cells. Here, the catalytic properties of PldA were characterized providing insights into possible molecular mechanisms by which PldA promotes cell invasion. Occurrences of multi-drug resistant bacterial infections are on the rise, however limited novel bactericidal agents are in development. Inhibition of virulence mechanisms is an emerging strategy to identify novel therapeutic targets for antibacterial therapy development. Presented here is a description of the first small-molecule inhibitor of a PLD virulence factor, a necessary preliminary step to confirm PldA inhibition as an efficacious strategy to treating P. aeruginosa infections.
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